Catalog Number - 85318-25P

The 85318‑25P Cogent RP C18 ABX™ HPLC Column is engineered for maximum‑separation analytical workflows, delivering robust, reproducible performance across extreme mobile phase conditions (pH 2–12).

Built with a bulky end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and enhances chromatographic performance for acidic, basic, hydrophobic, and neutral analytes, especially those with extreme pKa values.

✅ Ideal as a USP L1 alternate for methods involving strong acids or bases

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the 3 µm ABX analytical column range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and critical separations

• Provides extended retention and improved separation of difficult analytes

• Preferred for final method validation and highest‑purity results

✅ Selected when shorter columns (50 mm / 100 mm) cannot achieve required resolution

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development and optimization

  • QC and regulated workflows

  • final validation methods

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust, reproducible analytical performance

3 µm Particle – High Efficiency with Manageable Pressure

• Higher efficiency than 5 µm columns

• Produces:

  • Sharper peaks

  • Improved resolution

• Lower backpressure than 1.8 µm UHPLC columns

✅ Ideal for:

• High‑performance HPLC systems

• Maximum separation without UHPLC pressure requirements

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phases

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains performance under aggressive conditions

✅ Enables separations not achievable with standard C18 phases

Why Choose RP C18 ABX™ Columns (3 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for harsh analytical conditions and stability‑indicating methods

Improved Peak Shape for Acids & Bases

• Advanced end‑capping reduces secondary interactions

• Enhances reproducibility and accuracy

Maximum Separation Capability

• Designed for highest resolution analytical workflows

• Ideal for complex samples and regulatory methods

✅ One of the top choices for high‑resolution, extreme pH analytical methods

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Enables:

  • Improved separation of complex sample matrices

  • Enhanced resolution compared to shorter column formats

✅ Ideal for final method validation and high‑purity workflows

System & Sample Considerations

• Compatible with standard HPLC systems

• Can be used on UHPLC systems if available

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain long‑term performance and reproducibility

Typical Applications

• HPLC analytical separations

• Acidic and basic compound analysis

• Pharmaceutical method development and validation

• Stability‑indicating methods

• Complex mixture and impurity profiling

• High‑purity analytical workflows

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum‑resolution and highest‑purity workflows

• Monitor system pressure relative to instrument limits